Open Access Article
International Journal of Nursing Research. 2020; 2: (4) ; 463-465 ; DOI: 10.12208/j.ijnr.20200142.
Analysis of results of detection of HIV antibody by colloidal gold method and enzyme-linked immunoassay
采用胶体金法和酶联免疫法检测艾滋病病毒(HIV)抗体结果分析
作者：
于烻彬 *,
刘晓娜,
李周珣
云南省第二人民医院核医学科 云南昆明
*通讯作者：
于烻彬,单位：云南省第二人民医院核医学科 云南昆明；
发布时间: 2020-07-16 总浏览量: 800
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摘要
目的 探析在检测艾滋病病毒（HIV）抗体中采用胶体金法和酶联免疫法检测结果的分析。方法便利选取我院收集的1520份血清进行研究，本研究开展时间为2019年1月-2020年12月，所有的血清均使用胶体金法和酶联免疫法进行检测，并将相关检测结果进行详细的统计，同时比较两种检结果的阳性率。结果 根据对不同方法检测结果的分析得知，所有的血清使用胶体金法进行检测时，共有20分血清检测出HIV抗体阳性，而所有的血清使用酶联免疫法进行检测时，共有36分血清检出HIV抗体阳性，两种检测方法的阳性率分别为（1.36%）、（2.37%）。两种检测方法检测阳性率比较，酶联免疫法相对较高，差异有统计学意义（P＜0.05）。结论 本研究表明，在对艾滋病病毒的检测中使用酶联法检测具有较高的敏感性，因此，在对艾滋病病毒（HIV）抗体进行检测时，建议选择酶联法完成。
关键词： 艾滋病病毒(HIV)抗体；胶体金法；酶联免疫法；结果分析
Abstract
Objective: To analyze the results of colloidal gold method and enzyme-linked immunoassay in the detection of HIV antibodies. Method: It is convenient to select 1520 sera collected in our hospital for research. This study was conducted from January 2019 to December 2020. All the sera were tested using colloidal gold method and enzyme-linked immunoassay, and the relevant test results perform detailed statistics and compare the positive rates of the two test results. Results: According to the analysis of the test results of different methods, when all the sera were tested using the colloidal gold method, a total of 20 sera tested positive for HIV antibodies, and when all the sera were tested using the enzyme-linked immunoassay, a total of 36 serum tested positive for HIV antibodies, the positive rates of the two detection methods were (1.36%) and (2.37%). Comparing the positive rates of the two detection methods, the enzyme-linked immunoassay method is relatively high, and the difference is statistically significant (P <0.05). Conclusion : This study shows that the enzyme-linked method used in the detection of HIV has a high sensitivity. Therefore, when testing antibodies to HIV (HIV), it is recommended to choose the enzyme-linked method to complete.
Key words： HIV Antibody; Colloidal Gold Method; Enzyme-Linked Immunoassay; Results Analysis
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引用本文
于烻彬, 刘晓娜, 李周珣, 采用胶体金法和酶联免疫法检测艾滋病病毒(HIV)抗体结果分析[J]. 国际护理学研究, 2020; 2: (4) : 463-465.